| pCT Cloning Kit-Blunt end |  | | Code | BK0040-0020 | | Classification | CLONING | | Size | 20rxns | | Order |  |
| Print |
|
| | PRODUCT DETAIL |
| Description |
| |
The pCT cloning Kit-blunt end is convenient for the cloning of PCR products by using an overnight ligation step. It is suitable for use in Taq and other non-proofreading DNA polymerase PCR product cloning. |
| Features |
| |
- High Efficiency: Per ng vector could reach over 105 transformed colonies.
- Choice of ampicillin selection
- Easy blue/ white colony screening.
- M13 forward and reverse primer sites for DNA sequencing.
- Compelet component. The kit attached with ligation Premix, control insert DNA linear form vector.
|
| |
| Feature |
Position (bp) |
| Multiple cloning site (MCS) |
361-448 |
| IacZ -αpeptide |
469-113 |
| pUC origin |
903 |
| Ampicillin resistance gene |
2486-1629 |
| Primer binding sites: |
|
| M13 forward primer |
323-339 |
| M13 reverse primer |
481-465 |
|
|
|
