| Supplied form : |
| |
20mM Tris-HCl pH8.0, 50％glycerol,
100mM KCl, 0.1Mm EDTA, 1mM DTT, 0.5％Tween20, 0.5％NP-40
|
| |
|
| Description |
| |
VAS Taq is isolated and purified from an E.Coli. strain that carries the cloned DNA polymerase gene from Thermus aquqticus YT-1strain.
|
| |
|
| Purity(SDS-PAGE)＞99％ |
| |
|
| 10X VAS Taq PCR Buffer |
| |
Composition: Tris-HCl, KCl, (NH4)2SO4, 15mM MgCl2, pH8.3 . |
| |
|
| Unit definition |
| |
One unit is the amount of enzyme that will incorporate 10 nmoles of dNTPs into acid-insoluble products at 72℃ in 30 min. |
| |
|
| PCR performance test |
| |
Good performance of DNA amplification by PCR is confirmed by using 0.2U Taq will amplified 2.5 kb DNA fragment in a 50μl reaction volume.
|
| |
|
| General reaction mixture for PCR ( total 50μl ) |
| |
| VAS Taq (5 units/μl ) |
0.5μl |
| 10X Taq Buffer |
5.0μl |
| dNTPs(2.5mM) |
4.0μl |
| Template |
＜ 1μg |
| Primer 1 |
0.2~1.0μM |
| Primer 2 |
0.2~1.0μM |
| H2O |
upto 50μl |
|
| |
|
| PCR products |
| |
As most PCR product amplified with VAS Taq have one A added at 3`- terminus, the obtained PCR product can be directly used for cloning into T-vector. |
| |
|
| |
|
